Toxicity assessment of hydroxyapatite-coated mechanical heart valve embedded with collagen to vascular endothelial cells

doi:10.3969/j.issn.2095-4344.2013.51.018

Abstract

Abstract:

BACKGROUND: In early experiments, we prepared hydroxyapatite-coated mechanical heart valve embedded with collagen using impulse laser sediment method.
OBJECTIVE: To further analyze the histocompatibility and toxicity of hydroxyapatite-coated mechanical heart valve embedded with collagen.
METHODS: After passage, canine vascular endothelial cell suspension was inoculated onto the hydroxyapatite-coated mechanical heart valve embedded with collagen. One group was inoculated in 5% CO2, 37 ℃ incubator for 3 weeks static culture, and the other group was inoculated in 5% CO2, 37 ℃ incubator for 3 weeks spinner culture. Scanning electron microscope was used to observe cell attachment on the material. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to the proliferative capacity of vascular endothelial cells cultured with the hydroxyapatite-coated mechanical heart valve embedded with collagen.
RESULTS AND CONCLUSION: During the spinner culture, adherent cells were found on the surface of mechanical heart valve, and the cells distributed evenly and confluent at 21 days to cover the surface of the material. The number of adherent cells in the spinner culture was higher than that in the static culture. The cells during the static culture were aggregated and distributed irregularly. The mechanical heart valve exhibited no effects on the proliferation of canine vascular endothelial cells which grew well. These findings indicate that the hydroxyapatite-coated mechanical heart valve embedded with collagen exert no effect on proliferation of vascular endothelial cells, has no toxicity and has good biocompatibility.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, tissue engineering, heart valves, hydroxyapatites

CLC Number:

R318

Cheng Guang-cun, Li Chun-sheng, Yan Yu, Wang Lan, Yan Zhong-ya, Luo Le, Fang Xiao-dong, Tao Ru-hua. Toxicity assessment of hydroxyapatite-coated mechanical heart valve embedded with collagen to vascular endothelial cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(51): 8894-8900.

Figures/Tables 6

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